PHYSICOCHEMICAL PROPERTIES OF CRYSTALLINE CLOSTRIDIUM BOTULINUM TYPE A TOXIN
نویسندگان
چکیده
منابع مشابه
Physicochemical Properties of Crystalline Clostridium Botulinum Type a Toxin*
Interest in the chemical nature and mode of action of bacterial toxins has been renewed by the simultaneously announced crystallization of botulinal (1) and tetanal toxins (2) and the recent purification of several toxoids (3-5). Of these, crystalline Clostridium botulinurn type A toxin alone has been submitted to an apparently complete elementary and amino acid analysis (6), as well as to prel...
متن کاملPhysiocochemical properties of crystalline Clostridium botulinum type A toxin.
Interest in the chemical nature and mode of action of bacterial toxins has been renewed by the simultaneously announced crystallization of botulinal (1) and tetanal toxins (2) and the recent purification of several toxoids (3-5). Of these, crystalline Clostridium botulinurn type A toxin alone has been submitted to an apparently complete elementary and amino acid analysis (6), as well as to prel...
متن کاملPurification and properties of Clostridium botulinum type F toxin.
Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...
متن کاملproduction and crystallization of clostridium botulinum type a toxin
introduction: widespread medical usage of botulinum toxins has increased the need for the precise analysis of its biological activity. the aim of this study was production and crystallization of the botulinum type a toxins. methods: in this study, clostridium botulinum was grown and inoculated into the tripticase soya broth culture media, under anaerobic condition and toxin production was monit...
متن کاملThe elemental and amino acid composition of crystalline Clostridium botulinum type A toxin.
The isolation of Clostridium botulinurn type A toxin in crystalline form (1, 2) has led to an interest in its chemical composition. An apparently complete analysis of the pure crystalline toxin is presented in this paper. Preliminary studies have indicated that the toxin is a protein with an isoelectric point of pH 5.6, possessing the properties of a globulin (l-3). It is free from carbohydrate...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1948
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)51036-x